Some Ultrastructural Features of Micronuclei during Conjugation and Autogamy in Paramecium aurelia
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چکیده
منابع مشابه
Isolation and Partial Characterization of Macro- and Micronuclei from Paramecium Aurelia
A method was developed for the isolation of macro- and micronuclei from Paramecium aurelia. This method utilized ionic and nonionic detergents to rupture the intact cells, calcium ions and spermidine were employed to protect the nuclei, and the nuclei were purified by centrifugation. Macronuclei consisted of 22% DNA, 10% RNA, and 68% protein. Micronuclei were composed of 9% DNA, 11% RNA, and 80...
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A dominant gene Su(kau-2) partially suppresses the effect of an apparently unlinked recessive kau-2. Gene kau-2 blocks chemical induction of conjugation in Paramecium aurelia syngen 8 when solutions of acriflavine + either KCl or MgCl-2 are used. Wild-type cells are induced to conjugate in either solution. When cells homozygous for kau-2 also have gene Su(kau-2), they are still uninducible in t...
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Two methods have been devised for the isolation of large quantities of purified pellicles (cortical layers) of Paramecium aurelia. Pellicles isolated by both procedures, when examined by electron microscopy, were found to contain ciliary basal bodies, two types of cortical membranes, ribbons of microtubules, kinetodesmal fibers, and elements of the infraciliary lattice system. By electron micro...
متن کاملFactors Determining Conjugation in Paramecium Aurelia I. the Cyclical Factor: The Recency of Nuclear Reorganization.
N Paramecium aurelia a number of factors interact to determine the I occurrence of conjugation. Most attention has in the past been given to the role of environmental conditions. In this paper an internal factor is dealt with: nuclear reorganization of the type occurring both a t endomixis and a t conjugation. As will be shown, the relation of these nuclear processes to the subsequent occurrenc...
متن کاملFactors Determining Conjugation in Paramecium Aurelia II. Genetic Diversities between Stocks or Races.
N TYPICAL biotypes of the Johns Hopkins stock R of P. aurelia, I SONNEBORN (1936) has shown that, when surplus animals from isolation lines are collected in a small mass culture and placed a t 31OC, conjugation invariably occurs if the animals have recently been through a nuclear reorganization (that is, endomixis or conjugation), but never a t other times. In the present paper we contrast the ...
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ژورنال
عنوان ژورنال: Journal of General Microbiology
سال: 1976
ISSN: 0022-1287
DOI: 10.1099/00221287-94-1-193